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  • 02.08.2018
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Devitrification fish sperm

Center for Aquaculture Technologies, Inc. The Southern Flounder Paralichthys lethostigma is a high-value species and a promising aquaculture candidate. The goal of this work was to develop a standardized approach for vitrification of Southern Flounder sperm. The specific objectives were to 1 evaluate thawing methods and vitrification solutions, 2 evaluate the postthaw membrane integrity of sperm vitrified in different cryoprotectant solutions, 3 examine the relationship between membrane integrity and motility, and 4 evaluate the ability of vitrified sperm to fertilize eggs.
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Fish Sperm Physiology: Structure, Factors Regulating Motility, and Motility Evaluation

Devitrification fish sperm
Devitrification fish sperm
Devitrification fish sperm
Devitrification fish sperm
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Our goal was to develop a standardized approach for sperm vitrification of marine fishes that can be applied generally in aquatic species. The objectives were to: 1 estimate acute toxicity of cryoprotectants over a range of concentrations; 2 evaluate the properties of vitrification solutions VS ; 3 evaluate different thawing solutions, and 4 evaluate sperm quality after thawing by examination of motility and membrane integrity. Sperm were collected from red snapper Lutjanus campechanus , spotted seatrout Cynoscion nebulosus , and red drum Sciaenops ocellatus. A total of 29 combinations of cryoprotectants were evaluated for toxicity and glass formation.
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The path to success for fish sperm

Our goal was to develop a standardized approach for sperm vitrification of marine fishes that can be applied generally in aquatic species. The objectives were to: 1 estimate acute toxicity of cryoprotectants over a range of concentrations; 2 evaluate the properties of vitrification solutions VS ; 3 evaluate different thawing solutions, and 4 evaluate sperm quality after thawing by examination of motility and membrane integrity. Sperm were collected from red snapper Lutjanus campechanus , spotted seatrout Cynoscion nebulosus , and red drum Sciaenops ocellatus. A total of 29 combinations of cryoprotectants were evaluated for toxicity and glass formation. Adaptations by marine fish to high osmotic pressures could explain the survival in the high cryoprotectant concentrations.
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In many animals, males pursue alternative tactics when competing for the fertilization of eggs. Some cichlid fishes from Lake Tanganyika breed in empty snail shells, which may select for extremely divergent mating tactics. A recent study at the Institute of Ecology and Evolution of the University of Bern shows that different male types within a species produce divergent sperm, specializing either in speed or longevity.
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